Journal: Journal of Cell Science
Article Title: MOS is a key regulator of meiotic midbody assembly and prevents abnormal divisions in mouse eggs and their polar bodies
doi: 10.1242/jcs.264411
Figure Lengend Snippet: Mos −/− eggs have defects in meiotic midbody formation. (A–D) Representative immunofluorescence images of wild-type (A) and Mos −/− eggs (B–D) showing localization of MKLP1 (magenta), tubulin (green), and DNA (white). Arrowheads indicate structure features of MKLP1. Wild-type and Mos −/− oocytes were fixed 9–11.5 h after prophase I release. Scale bars: 10 µm. (E) Percentage of wild-type and Mos −/− eggs with normal or defective MKLP1 structure ( n indicates the number of eggs imaged per condition; three or more independent experiments conducted per condition). **** P <0.0001 (two-sided Fisher's exact test).
Article Snippet: The primary antibodies used were: rabbit anti-MKLP1 polyclonal antibody (Novus NBP2-56923; 1:100) and rabbit anti-PRC1 polyclonal antibody (Proteintech 15617-1-AP; 1:100).
Techniques: Immunofluorescence